Serrano began his acting career in off-Broadway plays in the late 1970s. His first film was the 1986 Tom Hanks comedy The Money Pit. Since then, he has appeared in supporting roles in films such as Lethal Weapon 2, Bad Boys, The Negotiator, Empire, The Insider, Runaway Jury, and The Day After Tomorrow. He also has an extensive television résumé, with appearances in shows such as Alias, 24, Law & Order, and its spin-offs, SVU and Trial by Jury.
Serrano played Bobby's father on an episode of Ugly Betty.
Nestor also played Victor, Adrianna Tate-Duncan's manager, on the CW show, 90210
He also made a guest-appearance in Criminal Minds in the episode, 'Poison' as Detective Hanover.
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